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ProteomicsMudPIT analyses of the proteins co-purified with the Interactor of Little Elongation Complex ELL subunit 1 (FLAG-ICE1), WT MED26, and D2G8 NTD-mutant MED26, affinity-purified from HEK293T cell lines
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the lit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
ProteomicsABIN1 is an signal-induced autophagy adaptor counteracting LUBAC-mediated linear ubiquitination
ABIN1 KO RAW264.7 cells and stably reconstituted with 3×FLAG-ABIN1 WT or D485N were subjected to mass spectrometric analysis. Three biological replicates were prepared for each condition. Cells were stimulated for 3 hours with 100 ng/ml LPS. ABIN1 was immunoprecipitated with 30 uL anti-FLAG M2 Magne...
ORGANISM(S): Mus Musculus (mouse) 
2022-02-17 | PXD024864 | JPOST Repository
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