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TranscriptomicsPromoter proximal pausing in response to stress in serum deprived mouse fibroblasts.
We have employed short-capped RNA sequencing (sc-RNA-seq) in order to identify genes whose expression is regulated by promoter proximal pausing of RNA Polymerase II (RNAPI) in response to stress stimulation. We used serum-deprived mouse Swiss 3T3 fibroblasts, either untreated (control) or treated wi...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-55775 | biostudies-arrayexpress
GenomicsStress-induced signaling to the nucleosome in serum deprived mouse fibroblasts.
We have employed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze changes in chromatin architecture as well as the occupancy of two RNA Polymerase II (RNAPII) isoforms, initiation-competent (RNAPIIS5ph) as well as elongation-competent (RNAPIIS2ph) upon stres...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-55774 | biostudies-arrayexpress
TranscriptomicsTranscriptional response to stress in serum deprived mouse fibroblasts in the presence of MSK1/2 inhibitor.
We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin for 3 or 6 hours to induce the p38/MAP kinase pathway. In order determine transcriptional effec...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-58746 | biostudies-arrayexpress
TranscriptomicsTranscriptional response to stress in serum deprived mouse fibroblasts [RNA-Seq]
We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway. Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-55776 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of stress response in mouse fibroblasts
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2014-08-11 | E-GEOD-55784 | biostudies-arrayexpress
TranscriptomicsTranscriptional response to stress in serum deprived mouse fibroblasts
We have employed gene expression profiling in order to identify targets of transcriptional response to stress in mouse Swiss 3T3 fibroblasts, where we induced p38/MAP kinase pathway using anisomycin. Serum starved (72 h) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1, 3, and 6 h (in t...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-55554 | biostudies-arrayexpress
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