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TranscriptomicsRNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
The transcriptome of splenic classical dendritic cells (cDCs) (CD3- CD19- CD45+ MHC-II+ CD11c+) was compared in Wild Type (WT) mice, Germ Free (GF) mice and mice deficient for the Ifnar1 gene (IFNAR). Cells were isolated and sorted by flow cytometry to high purity. The cells were stimulated ex vivo ...
ORGANISM(S): Mus musculus 
2018-06-30 | E-MTAB-5190 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of primary isolated unstimulated murine splenic DC1 and DC2 from specific pathogen free (SPF), Germ free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice
The transcriptomes of primary isolated unstimulated murine splenic cDC1 and cDC2 from specific pathogen free (SPF) were compared to cDC1 and cDC2 from Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice. Total RNA was prepared from sort-purified cDC1 and cDC2 (for each sample ...
ORGANISM(S): Mus musculus 
2020-03-30 | E-MTAB-8883 | biostudies-arrayexpress
GenomicsChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
The epigenetic state of splenic cDCs from Wild Type (WT) mice, Germ Free (GF) and mice deficient for the Ifnar1 gene (IFNAR) was characterized by analyzing the occupancy of a major activating histone mark (H3K4me3) and a major repressive histone mark (H3K27me3). Splenic cDCs (CD3- CD19- CD45+ MHC-II...
ORGANISM(S): Mus musculus 
2018-06-30 | E-MTAB-5234 | biostudies-arrayexpress
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