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MetabolomicsA combined direct infusion/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards
We propose a fully automated novel workflow for lipidomics based on flow injection, followed by liquid chromatography-high-resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome achieved via reversed-phase LC-HRMS with absolute quantification by us...
2021-07-01 | MTBLS1876 | MetaboLights
MetabolomicsBenchmarking non-targeted metabolomics using yeast extract controls

Non-target analysis based on high resolution mass spectrometry calls for quality controls to validate analytical workflows. So far no community-wide used quality control material has been established in metabolomics. In this work, we propose ethanolic yeast extracts derived from Pichia pastoris i...

2020-09-29 | MTBLS1782 | MetaboLights
MetabolomicsWhat CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell lines

Background: Cell line specific, genome-scale metabolic models enable rigorous and systematic in silico investigation of cellular metabolism. Such models have recently become available for Chinese hamster ovary (CHO) too. However, a key ingredient, namely an experimentally validat...

2020-07-13 | MTBLS1210 | MetaboLights
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