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TranscriptomicsThe mRNA-bound proteome and its global occupancy profile on protein-coding transcripts [PAR-CLIP]
Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteom...
ORGANISM(S): Homo sapiens 
2012-06-01 | E-GEOD-38201 | biostudies-arrayexpress
TranscriptomicsThe mRNA-bound proteome and its global occupancy profile on protein-coding transcripts [RNA-seq]
Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteom...
ORGANISM(S): Homo sapiens 
2012-06-01 | E-GEOD-38356 | biostudies-arrayexpress
TranscriptomicsMOV10 is a 5' to 3' RNA Helicase Contributing to UPF1 mRNA Target Degradation by Translocation Along 3' UTRs (PAR-CLIP)
RNA helicases are important regulators of gene expression that act by remodeling RNA secondary structures and as RNA-protein interactions. Here, we demonstrate that MOV10 has an ATP-dependent 5' to 3' in vitro RNA unwinding activity and determine the RNA-binding sites of MOV10 and its helicase mutan...
ORGANISM(S): Homo sapiens 
2014-04-11 | E-GEOD-48245 | biostudies-arrayexpress
TranscriptomicsThe mRNA-bound proteome and its global occupancy profile on protein-coding transcripts [protein occupancy profiling]
Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteom...
ORGANISM(S): Homo sapiens 
2012-06-01 | E-GEOD-38355 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse HL1, C2C12 differentiated and C2C12 undifferentiated cells with four histone modifications
The combinatorial effects of four histone modifications in transcription and differentiation. Two replicates per histone modification and cell type.
ORGANISM(S): Mus musculus 
2007-10-17 | E-TABM-182 | biostudies-arrayexpress
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