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In order to assess the transcriptional modifications of dendritic cells treated with Denileukin diftitox (ONTAK), we profiled the whole transcriptome of monocyte-derived dendritic cells (moDC) from blood of healthy volounteers after stimulation with a maturation cocktail with or without the addition...
ORGANISM(S): Homo sapiens 
In this experiment, dendritic cells and monocytes obtained from melanoma patients who underwent immunotherapy were stimulated with a maturation cocktail then either treated or not treated with denlieukin diftitox (DD; ONTAK) and then subjected to transcription profiling to investigate the effects of...
ORGANISM(S): Homo sapiens 
Data Access Committee EGAC00001000064
SNP 6.0 arrays of small cell lung cancer
Whole genome sequencing was performed with DNA extracted from fresh-frozen tumor and normal material. Short insert DNA libraries were prepared with the TruSeq DNA PCRfree sample preparation kit (Illumina) for paired-end sequencing at a minimum read length of 2x100bp. Human DNA libraries were sequenc...
RNA-sequencing (RNA-seq) was performed with RNA extracted from fresh-frozen human tumor tissue samples. cDNA libraries were prepared from poly-A selected RNA applying the Illumina TruSeq protocol for mRNA. The libraries were then sequenced with a 2 x 100bp paired-end protocol to a minimum mean cover...
We have sequenced the genomes of 110 small cell lung cancers (SCLC), one of the deadliest human cancers. We found bi-allelic inactivation of TP53 and RB1 in nearly all the tumors analyzed, sometimes by complex genomic rearrangements. Two tumors with wild-type RB1 had evidence of chromothripsis leadi...
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