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ProteomicsPhos-tag SDS-PAGE followed by LC-MS/MS analysis of in vitro phosphorylated Nab2, Foxk1, and HURP
Purified GST-fused Nab2, Foxk1, and HURP were incubated with ATP in the presence or absence of His-tagged active ERK in vitro, separated by Phos-tag SDS-PAGE, and visualized by CBB staining. The most shifted regions of protein bands on the Phos-tag-containing gels were excised, digested with trypsin...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028837 | JPOST Repository
ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
Proteomics1DE-MS profiling of rat cerebral cortex proteins of focal cerebral ischemia-reperfusion injury by combining SDS-PAGE, gel slicing, quantitative LC-MS/MS and profile reconstruction
A rat model of focal cerebral ischemia-reperfusion (I/R) injury was established by 2 hour-middle cerebral artery occlusion (MCAO) followed with 1, 7 and 14 days of reperfusion. Validation of the model was done by the measurement on cerebral blood flow, infarct volume, histological damage and neurol...
ORGANISM(S): Rattus Norvegicus 
2022-05-11 | PXD033793 |
ProteomicsBAC-DROP: Rapid digestion of proteome fractionated via dissolvable polyacrylamide gel electrophoresis and its application to bottom-up proteomics workflow
The GeLC-MS workflow, which combines low-cost, easy-to-use SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with liquid chromatography-mass spectrometry (LC-MS), is very popular in current bottom-up proteomics. However, GeLC-MS requires that PAGE-separated proteins undergo overnight enzymatic diges...
ORGANISM(S): Homo Sapiens Hepatitis B Virus Bos Taurus 
2021-01-24 | PXD021489 | panorama
UnknownRoast-Driven Coffee Proteome Changes Characterized by Bradford Assay, SDS-PAGE, and LC-MS.
Not available
S-EPMC12896866 | biostudies-literature
ProteomicsHuman placental tissue LC-MS/MS
This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, mic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-29 | MSV000086385 | MassIVE
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-29 | MSV000081772 | MassIVE
ProteomicsHuman testis off-line LC-MS/MS
In order to identify more MPs and get deeper proteomic data of testis. We applied high-pH reverse phase (RP) HPLC to fractionate complex samples.On the other hand, to enhance protein coverage, multi-protease strategies were used for 10% SDS-PAGE short separation samples.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-19 | MSV000086491 | MassIVE
UnknownFast and high-throughput LC-MS characterization, and peptide mapping of engineered AAV capsids using LC-MS/MS.
Not available
S-EPMC9563341 | biostudies-literature
ProteomicsPRM analysis of phosphopeptides of Nab2, Foxk1, HURP, ERK1/2, NPM, and Hspa4 from ΔB-Raf:ER cells.
ΔB-Raf:ER cells were treated with U0126 or 4-HT for 30 min in four biological replicates. Total proteins from cell lysates were digested with Typsin/Lys-C mix, and phosphopeptides were enriched by IMAC using Fe-NTA columns. After data-dependent LC-MS/MS analysis, phosphopeptides of Nab2, Foxk1, HURP...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028826 | JPOST Repository
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