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TranscriptomicsRNA-seq of NP peptide stimulated F5 huCD2iCre Ikk2fx/fx CD8+ T cells
Cells were isolated from lymph nodes of F5 Rag1-/- Cre -ve and F5 Rag1-/- huCD2iCre Ikk2fx/fx mice and cultured for 24hrs with NP-68 peptide, necrostatin-1 and splenocytes from Rag1-/- mice. A pure population of CD8+ T cells was sorted by FACS and the RNA extracted.
ORGANISM(S): Mus musculus 
2024-09-30 | E-MTAB-14348 | biostudies-arrayexpress
TranscriptomicsRNA-seq of CD8 single positive thymocytes from WT and Rela fx/fx CD4Cre Nfkb1KO RelKO mice.
Gene expression by CD8 thymocytes was compared between cells from WT mice, and cell from mice with gremlin deletions of cRel and Nfkb1, and a T cell specific deletion of Rel using CD4 Cre.
ORGANISM(S): Mus musculus 
2019-08-23 | E-MTAB-7470 | biostudies-arrayexpress
TranscriptomicsRNA-seq of total lymph node cells from inducible ll7 gene deletion mice
Il7fx/KO Rosa26CreERT2 mice (samples 4, 5 and 6) and CreERT –ve littermate controls (samples 1, 2 and 3) were treated with tamoxifen for five days. Three weeks later, RNA was isolated from total lymph nodes of individual mice and gene expression determined by RNAseq.
ORGANISM(S): Mus musculus 
2018-02-16 | E-MTAB-6451 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of mouse CD8+ naive T cells from mice expressing normal IL-7R or conditional IL-7R alpha switched on or off for different amounts of time
The transcriptional profile of CD8+ naive T cells was compared from F5 transgenic mice expressing normal IL-7R or a conditional IL-7R alpha that was either switched on, or switched off for different amounts of time.
ORGANISM(S): Mus musculus 
2010-12-20 | E-TABM-991 | biostudies-arrayexpress
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