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TranscriptomicsAnalysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq
Using a chemical treatment, the sodium periodate (NaIO4) /β-Elimination oxidation, and then performing deep-sequencing of small RNAs with NGS technologies, we evaluated the methylation status of the piRNAs population in the metastatic COLO 205 cell line.
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8115 | biostudies-arrayexpress
TranscriptomicsRNA-Immunoprecipitation of PIWIL1/piRNA complexes in COLO 205 cells and deep-sequencing of associated small RNAs with NGS technologies
To evaluate PIWIL1/piRNAs association in the metastatic COLO 205 cell line, we performed an RNA-immunoprecipitation experiment followed by small RNA deep-sequencing (RIP-Seq). For the identification of PIWIL1/piRNA complexes, independent reactions were carried out with two different antibodies (IP1 ...
ORGANISM(S): Homo sapiens 
2020-08-05 | E-MTAB-8121 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of total RNA in the COLO 205 cell line with Next-Generation Sequencing (NGS) technologies
The transcriptome of COLO 205 cells was investigated using an RNA-Seq approach. This allowed us to evaluate the expression of mRNAs encoding for the major interactors of PIWIL proteins and factors involved in piRNAs biogenesis.
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8116 | biostudies-arrayexpress
TranscriptomicsExpression profiles of piRNAs in Colorectal Cancer (CRC) cell lines
To investigate the presence and abundance of piRNAs in Colorectal Cancer (CRC), we performed deep-sequencing of the small RNA transcriptome of eight human CRC cell lines (HT-115, Caco-2, SW-1417, SW 403, COLO 205, HT-29, HCT 116 and RKO).
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8117 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of human breast cancer cells MCF7 and LCC2 treated with Tamoxifen; EPZ or DMSO
RNA-Seq of human breast cancer cells MCF7 and LCC2 treated with Tamoxifen; EPZ or DMSO
ORGANISM(S): Homo sapiens 
2019-10-11 | E-MTAB-6892 | biostudies-arrayexpress
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