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TranscriptomicsRNA-Seq of monoclonal cell lines after CRISPR/Cas9-mediated editing of box C/D snoRNA genes (SNORDs)
In this study monoclonal cell lines carrying mutations in snoRNA genes (SNORD74, SNORD75, SNORD77, SNORD80) were obtained. Next, whole transcriptome analysis of obtained cell lines was performed on an Illumina NextSeq 500 platform. RNA-seq libraries was constructed on PolyA mRNA-enriched fraction. T...
ORGANISM(S): Homo sapiens 
2019-10-14 | E-MTAB-8269 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected MRC5, WI-38 VA-13, A549 and HEK293FT human cells
To study genes involved in cellular response to Influenza A virus infection, human cells MRC5, WI-38 VA-13, A549 and HEK293FT were infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. RNA-seq analysis of infected cell lines after 48 h was performed on an Illumina NextSeq 500 platform. The same...
ORGANISM(S): Homo sapiens 
2020-11-23 | E-MTAB-9511 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of monoclonal cell lines after CRISPR/Cas9-mediated editing of the small nucleolar RNA host-gene GAS5
In this study monoclonal cell lines carrying mutations in the small nucleolar RNA host-gene GAS5 were obtained. Next, Poly(A) RNA-seq and Small RNA-seq analyses of obtained cell lines were performed on an Illumina NextSeq 500 platform. Poly(A) RNA-seq libraries was constructed on polyA mRNA-enriched...
ORGANISM(S): Homo sapiens 
2023-11-14 | E-MTAB-13515 | biostudies-arrayexpress
TranscriptomicsSmall RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected A549 human cells
To study expression pattern of small nucleolar RNAs (snoRNAs) during influenza A viral infection, human cells A549 were infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Small RNA-seq analysis of infected cells after 24 h or 48 h incubation was performed on an Illumina NextSeq 500 platform....
ORGANISM(S): Homo sapiens 
2022-11-01 | E-MTAB-12088 | biostudies-arrayexpress
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