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ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
UnknownIdentification of myo-inositol hexakisphosphate (IP6) as a β-secretase 1 (BACE1) inhibitory molecule in rice grain extract and digest.
Not available
S-EPMC3953730 | biostudies-literature
Methylation profilingDigital Restriction Enzyme Analysis of Methylation (DREAM) of normal colon genomic DNA.
We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput...
ORGANISM(S): Homo sapiens 
2017-02-08 | GSE83477 | GEO
Methylation profilingDigital Restriction Enzyme Analysis of Methylation (DREAM) of SW48 colon cancer cell line genomic DNA.
We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput...
ORGANISM(S): Homo sapiens 
2015-09-08 | GSE66296 | GEO
Methylation profilingDNA methylation drift as a marker of lifespan in mammals [monkey]
We analyzed levels of 5-methyl cytosine at CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput s...
ORGANISM(S): Macaca mulatta 
2017-07-10 | GSE75496 | GEO
Methylation profilingDigital Restriction Enzyme Analysis of Methylation (DREAM) of normal breast epithelial cells and MCF7 genomic DNA
We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput...
ORGANISM(S): Homo sapiens 
2017-02-08 | GSE74910 | GEO
Methylation profilingDigital Restriction Enzyme Analysis of Methylation (DREAM) of HL60 genomic DNA
We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput...
ORGANISM(S): Homo sapiens 
2017-02-08 | GSE78103 | GEO
Methylation profilingDNA methylation profiling in acute myeloid leukemia
We analyzed levels of 5-methyl cytosine CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput sequ...
ORGANISM(S): Homo sapiens 
2019-06-06 | GSE82288 | GEO
Methylation profilingDNA methylation drift as a marker of lifespan in mammals [mouse]
We analyzed levels of 5-methyl cytosine at CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput s...
ORGANISM(S): Mus musculus 
2017-07-10 | GSE75495 | GEO
Methylation profilingDNA methylation drift as a marker of lifespan in mammals [human]
We analyzed levels of 5-methyl cytosine CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput sequ...
ORGANISM(S): Homo sapiens 
2017-07-10 | GSE75274 | GEO
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