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TranscriptomicsHuman umbilical cord blood-derived endothelial colony forming cells under serum-free conditions
Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum ...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress
MetabolomicsCurcumin (analogue) metabolites in intestinal cell lines
Curcumin and curcumin-based benzothiazepane analogues were investigated in intestinal cell lines of cancer (HCT-116) and non-cancer (IPEC-J2) origin. After 0h, 3h and 15h incubation, the cell lysates and extracellular media were analysed using LC-QTOF-MS.
2026-07-20 | MTBLS15099 | MetaboLights
MetabolomicsSingle cell lipidomics of adherent gut epithelial cell lines Caco2 and HT29-MTX reveals a metabolic switch

Raw MS data files for the CaCo02 and HT29-MTX single cells isolated using the Yokogawa SS2000 for the research article 'Single cell lipidomics of adherent gut epithelial cell lines Caco2 and HT29-MTX reveals a metabolic switch' 

2026-03-18 | MTBLS14074 | MetaboLights
MetabolomicsMultiomic analysis reveals that polyamines alter G. vaginalis-induced cervicovaginal epithelial cell dysfunction

An anaerobe-dominant, Lactobacillus-deplete cervicovaginal microbiome is associated with adverse reproductive outcomes. Gardnerella vaginalis, a cervicovaginal anaerobe, alters cervicovaginal epithelial cell function, resulting in immune activation and barrier breakdow...

2026-02-10 | MTBLS13885 | MetaboLights
TranscriptomicsGene expression profiles during the establishment of CHK-Q (Chinese hamster kidney-derived cell line) and derivative cells
CHK-Q cell line was established from primary culture of Chinese hamster kidney tissues by repeated passages. DNA microarray analysis for chasing the variation in gene expression profile was performed for CHK cells during the culture process leading to immortalization and adaptation to serum-free and...
ORGANISM(S): Cricetulus griseus 
2022-03-16 | E-MTAB-11298 | biostudies-arrayexpress
MetabolomicsSingle-cell lipidomic analysis of the Epithelial-Mesenchymal Transition (EMT) using MALDI-MSI

EMT is a metastasis-promoting process whose heterogeneity has been extensively studied at a gene expression level. EMT also involves reprogramming of lipid metabolism, however there has been little focus on heterogeneity on the lipid level. Here, we use MALDI-MSI to determine the levels of variou...

2026-04-15 | MTBLS12650 | MetaboLights
ProteomicsLabel-free shotgun proteomics of Mycoplasma pneumoniae grown in serum-free medium
We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we analyzed the proteome of the wild-type strain grown in serum-free vB13 medium compared to rich medium (Hayflick medium).
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) Bacteria 
2023-04-05 | PXD037431 | Pride
Mycoplasma pneumoniae Serum-free medium 
MetabolomicsCell of origin epigenetic priming determines susceptibility to Tet2 mutation

Hematopoietic stem cell (HSC) mutations can result in clonal hematopoiesis (CH) with heterogeneous clinical outcomes. Here, we investigated how the cell state preceding Tet2 mutation impacts the pre-malignant phenotype. Using an inducible system for clonal analysis of myeloid progenitors...

2024-04-30 | MTBLS9914 | MetaboLights
GenomicsFatty acid biosynthesis is insufficient in HSCs cultured in serum-free culture conditions
Fatty acid biosynthesis is insufficient in HSCs cultured in serum-free culture conditions
PRJNA482376 | ENA
ProteomicsAntibody-free multiplex measurement of 23 human cytokines in primary cell culture secretome using targeted multiple reaction monitoring mass spectrometry
Cytokines are key mediators of inflammation that commonly serve as biomarkers to monitor immune responses and inflammatory disease. Cytokines are commonly measured by immunoassays, which have limited multiplexing capacity, are costly and can exhibit cross-reactivity. Although multiple reaction monit...
ORGANISM(S): Homo Sapiens 
2020-02-19 | PXD015448 | panorama
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