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TranscriptomicsHuman umbilical cord blood-derived endothelial colony forming cells under serum-free conditions
Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum ...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress
ProteomicsLabel-free shotgun proteomics of Mycoplasma pneumoniae grown in serum-free medium
We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we analyzed the proteome of the wild-type strain grown in serum-free vB13 medium compared to rich medium (Hayflick medium).
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) Bacteria 
2023-04-05 | PXD037431 | Pride
Mycoplasma pneumoniae Serum-free medium 
TranscriptomicsComparative gene expression profiling of serum-free and FBS treated colorectal TIC cultures
To induce a differentiated phenotype, primary colorectal TIC cultures were grown in 10% FBS containing conditions. To analyze gene expression changes upon induction of a differentiated phenotype, total RNA of cells cultured in FBS containing conditions and control cells cultured under serum-free co...
ORGANISM(S): Homo sapiens 
2016-08-20 | E-GEOD-72543 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Mycoplasma pneumoniae grown in serum-free medium
We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we performed RNA-seq expression analysis of the wild-type strain grown in serum-free vB13 medium.
ORGANISM(S): Mycoplasma pneumoniae 
2023-04-03 | E-MTAB-12308 | biostudies-arrayexpress
TranscriptomicsAscorbate effect on serum free cultured hESC
Ascorbate activates CD30 expression and causes widespread specific demethylation of the epigenome of serum free cultured hESC. The genetic and epigenetic integrity of human Embryonic Stem cells (hESCs) is critical to their future applications in research and medicine. hESC cultured in serum free me...
ORGANISM(S): Homo sapiens 
2010-06-23 | E-GEOD-16590 | biostudies-arrayexpress
GenomicsFatty acid biosynthesis is insufficient in HSCs cultured in serum-free culture conditions
Fatty acid biosynthesis is insufficient in HSCs cultured in serum-free culture conditions
PRJNA482376 | ENA
TranscriptomicsComparative gene expression profiling of serum-free and FBS treated adherent primary pancreatic TIC cultures
To induce a differentiated phenotype, primary pancreatic TIC cultures were grown in 10% FBS containing conditions. To analyze gene expression changes upon induction of a differentiated phenotype, total RNA of cells cultured in FBS containing conditions and parallel control cells cultured under serum...
ORGANISM(S): Homo sapiens 
2017-05-16 | E-GEOD-59118 | biostudies-arrayexpress
TranscriptomicsGene expression profiles during the establishment of CHK-Q (Chinese hamster kidney-derived cell line) and derivative cells
CHK-Q cell line was established from primary culture of Chinese hamster kidney tissues by repeated passages. DNA microarray analysis for chasing the variation in gene expression profile was performed for CHK cells during the culture process leading to immortalization and adaptation to serum-free and...
ORGANISM(S): Cricetulus griseus 
2022-03-16 | E-MTAB-11298 | biostudies-arrayexpress
TranscriptomicsGene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium
Human umbilical cord mesenchymal stem cells maintained multipotency and immunosuppressive ability when being cultured in chemical defined serum free medium, but gained different gene expression profile. We used microarrays to identify the transcriptional difference between human umbilical cord mesen...
ORGANISM(S): Homo sapiens 
2014-10-28 | E-GEOD-62665 | biostudies-arrayexpress
Genomicsadaptation of NK-92 cells to serum free culture conditions
Functional assessment for clinical use of serum-free adapted NK-92 cells
PRJEB33549 | ENA
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