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TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of global gene expression changes during mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Microarray data analysis Intensity of 21,939 gene features per array was extracted from scanned microarray images using Feature Extraction 5.1.1 software (Agilent Technologies), which performs background subtractions and dye normalization. This normalization method is targeted at detecting changes i...
ORGANISM(S): Mus musculus 
2004-01-30 | E-MEXP-73 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Feature Extraction 5.1.1 software (Agilent Technologies) performs background subtractions and dye normalization. This normalization method is targeted at detecting changes in relative expression of individual genes rather than global expression. Global expression change would require external normal...
ORGANISM(S): Mus musculus 
2004-01-12 | E-MEXP-74 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells on a novel microarray design to validate the system
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-66 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse ES cell embrioid bodies treated with retinoic acid
Neural induction of ES by Retinoic acid
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-63 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryo and E12.5 placenta total RNA pooled to create 25:75, 50:50, and 75:25 ratio mixtures to estimate transcript copy number
E12.5 mouse whole embryo and E12.5 placenta total RNA were pooled to create 25:75, 50:50, and 75:25 ratio mixtures, based on Bioanalyzer quantitation. These samples, along with the original unmixed RNAs, were used as templates for duplicate linear amplification labeling reactions. cRNA target mixtu...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-67 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse ES cell neural induction (N2B27 monolayer cluture)
Neural induction of ES by N2B27 monolayer culture
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-64 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse ES, EC, NS, TS, placenta and neural induction (N2B27) comaprison
Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction.
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-65 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-69 | biostudies-arrayexpress
TranscriptomicsDefining a developmental path to neural fate by global expression profiling of mouse ESCs and adult NS cells
Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells. This SuperSeries is composed of the following subset Series: GSE4075: Neural induction (Embrioid bodies-Retinoic acid) GSE4076: Neural induction (N2B27 m...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-4082 | biostudies-arrayexpress
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