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TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsDefining a developmental path to neural fate by global expression profiling of mouse ESCs and adult NS cells
Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells. This SuperSeries is composed of the following subset Series: GSE4075: Neural induction (Embrioid bodies-Retinoic acid) GSE4076: Neural induction (N2B27 m...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-4082 | biostudies-arrayexpress
TranscriptomicsTranscript copy number estimation using a mouse whole-genome oligonucleotide microarray (22k Linearity)
E12.5 mouse whole embryo and E12.5 placenta total RNA were pooled to create 25:75, 50:50, and 75:25 ratio mixtures, based on Bioanalyzer quantitation. These samples, along with the original unmixed RNAs, were used as templates for duplicate linear amplification labeling reactions. cRNA target mixtu...
ORGANISM(S): Mus musculus 
2010-06-09 | E-GEOD-3508 | biostudies-arrayexpress
TranscriptomicsTranscript copy number estimation using a mouse whole-genome oligonucleotide microarray (WGA 2.1 Spike Test)
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-10-28 | E-GEOD-3509 | biostudies-arrayexpress
TranscriptomicsNeural induction (Embrioid bodies-Retinoic acid)
Neural induction of ES by Retinoic acid Neural induction of ES by Retinoic acid
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-4075 | biostudies-arrayexpress
TranscriptomicsIdentification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algor
We present a microarray experiment with the same tet-inducible system but with multiple time points within 24 hr to capture early responses to Oct4 suppression. These data were analyzed together with published genome-wide ChIP data. We found that most tentative target genes (TTG) of Oct4 in ES cells...
ORGANISM(S): Mus musculus 
2008-04-24 | E-GEOD-8617 | biostudies-arrayexpress
TranscriptomicsXenopus laevis embryos and animal caps: Control vs PouV morpholino
Transcriptional profiling of Xenopus laevis embryos and ectoderm (animal caps) comparing embryos injected with control morpholino with embryos injected with the morpholino mixture PVD2, which knocks down all three Xenopus PouV proteins. Whole embryos (WE) or animal caps (AC) were collected at late b...
ORGANISM(S): Xenopus laevis 
2013-11-12 | E-GEOD-47578 | biostudies-arrayexpress
TranscriptomicsNeural induction (N2B27 monolayer cluture)
Neural induction of ES by N2B27 monolayer culture Neural induction of ES by N2B27 monolayer culture
ORGANISM(S): Mus musculus 
2006-01-22 | E-GEOD-4076 | biostudies-arrayexpress
TranscriptomicsES, EC, NS, TS, Placenta and Neural induction (N2B27) comaprison
Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction. Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction.
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-4077 | biostudies-arrayexpress
TranscriptomicsDissecting Oct3/4-regulated gene networks in embryonic stem cells by expression
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation ass...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-5936 | biostudies-arrayexpress
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