Sort   by:  
 Page size 
TranscriptomicsBAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse ES cells
We used BAF250b-/- ES cells, two independently derived clones, at 18 and 72 hr in culture and compared them with parental cell line (wild-type) at the same time in culture (two replications each). Total RNAs were extracted using Trizol (Invitrogen) according to manufacturer protocol. 2.5 ug of total...
ORGANISM(S): Mus musculus 
2008-06-09 | E-GEOD-8996 | biostudies-arrayexpress
TranscriptomicsIdentification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algor
We present a microarray experiment with the same tet-inducible system but with multiple time points within 24 hr to capture early responses to Oct4 suppression. These data were analyzed together with published genome-wide ChIP data. We found that most tentative target genes (TTG) of Oct4 in ES cells...
ORGANISM(S): Mus musculus 
2008-04-24 | E-GEOD-8617 | biostudies-arrayexpress
TranscriptomicsDatabase for mRNA Half-Life of 19,977 Genes Obtained by DNA Microarray Analysis of Pluripotent and Differentiating Mouse
Degradation of mRNA is one of the key processes that control the steady-state level of gene expression. However, the rate of mRNA decay for the majority of genes is not known. We successfully obtained the rate of mRNA decay for 19 977 non-redundant genes by microarray analysis of RNA samples obtaine...
ORGANISM(S): Mus musculus 
2010-05-16 | E-GEOD-13609 | biostudies-arrayexpress
TranscriptomicsGlobal gene expression profiling reveals similarities and differences among mouse pluripotent stem cells
We present a compendium DNA microarray analysis of multiple mouse ESCs and EGCs from different genetic backgrounds (strains 129 and C57BL/6) cultured under standard conditions and in differentiation-promoting conditions by the withdrawal of Leukemia Inhibitory Factor (LIF) or treatment with retinoic...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-5914 | biostudies-arrayexpress
TranscriptomicsDefining Developmental Potency and Cell Lineage Trajectories by Expression Profiling of Differentiating Mouse ES Cells
Biologists rely on morphology, function, and specific markers to define the differentiation status of cells. Transcript profiling has expanded the repertoire of these markers by providing the snapshot of cellular status that reflects the activity of all genes. However, such data have been used only ...
ORGANISM(S): Mus musculus 
2008-12-27 | E-GEOD-11523 | biostudies-arrayexpress
TranscriptomicsRejuvenation of pluripotent stem cells by frequent activation of Zscan4, Part A
The gold standard for examining pluripotency of stem cells is to see whether cells can contribute to entire body of animals. Here we show that the increased frequency of Zscan4 activation in mouse ES cells not only enhances, but also maintains their developmental potency in long-term cell culture. A...
ORGANISM(S): Mus musculus 
2013-06-12 | E-GEOD-15604 | biostudies-arrayexpress
TranscriptomicsRejuvenation of pluripotent stem cells by frequent activation of Zscan4, Part B
The gold standard for examining pluripotency of stem cells is to see whether cells can contribute to entire body of animals. Here we show that the increased frequency of Zscan4 activation in mouse ES cells not only enhances, but also maintains their developmental potency in long-term cell culture. A...
ORGANISM(S): Mus musculus 
2013-06-12 | E-GEOD-26162 | biostudies-arrayexpress
TranscriptomicsRejuvenation of pluripotent stem cells by frequent activation of Zscan4
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2013-06-12 | E-GEOD-26278 | biostudies-arrayexpress
TranscriptomicsPrincipal components of embryonic stem cell pluripotency
To decipher gene regulatory networks, we used systematic suppression of 97 transcription factors (TFs) and 7 other genes with shRNA in mouse embryonic stem (ES) cells, followed by global gene expression profiling. Meta-analysis of these data together with the earlier data obtained by the induction o...
ORGANISM(S): Mus musculus 
2013-03-14 | E-GEOD-26520 | biostudies-arrayexpress
TranscriptomicsTimed induction of 50 transcription factors in ES cells reveals a common mechanism to initiate differentiations
To decipher the structure and behaviors of the transcription factor (TF) network, we created 50 permanent mouse ES cell lines, in each of which one of the 50 transcription factors tagged with FLAG, is inserted into the doxycycline (dox)-repressible ROSA26 locus. Expression profiling reveals Cdx2 as ...
ORGANISM(S): Mus musculus 
2009-10-01 | E-GEOD-14559 | biostudies-arrayexpress
Sort   by:  
 Page size