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ProteomicsEvaluation of absorbent cotton for glycopeptide enrichment
This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the perf...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-04 | PXD035078 | Pride
Hilic Glycopeptide Enrichment absorbent cotton Mass spectrometry Max 
ProteomicsPrecision mapping of glycosite-specific glycans reveals distinctive N-glycosylation on human spermatozoa
In this study, we applied the recently developed software, StrucGP to large-scale characterize glycoproteins, and IGPs, as well as precise site-specific glycan structures in human spermatozoa. Furthermore, bioinformatical analyses were conducted to systematically describe the potential functions of ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD026649 | Pride
Spermatozoa Glycan structures Mass spectrometry 
ProteomicsPrecision structural interpretation of site-specific N-glycans in seminal plasma
This study is aimed to comprehensively and globally characterize seminal plasma glycoproteins in a site-specific manner with information of N-glycosites and corresponding glycan structures, as well as glycan isomers using mass spectrometry. Furthermore, the function of specific glycan structures is ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD030804 | Pride
Seminal plasma Glycoproteomics Glycan structures Mass spectrometry Intact glycopeptides 
ProteomicsProfiling of human spermatozoa glycoproteins by LC/MS/MS
N-linked glycoproteins are known to be important for spermatozoa development and gametes fusion. However, little is known about detailed information of these glycoproteins, particularly the glycosylation sites and its corresponding glycan structures in human spermatozoa. In present study, an in hous...
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD020286 | Pride
Human Lc/ms/ms Glycan structures Spermatozoa 
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