Sort   by:  
 Page size 
GenomicsA highly sensitive and robust method for genome-wide 5hmC profiling of rare cell populations
Investigations of 5-hydroxymethylcytosine (5hmC) in biologically and clinically samples and models with low cell numbers have been hampered by the low sensitivity and reproducibility using current 5hmC mapping approaches. Here, we develop a selective 5hmC chemical labeling approach using tagmentatio...
ORGANISM(S): Mus musculus 
2016-09-01 | E-GEOD-77967 | biostudies-arrayexpress
TranscriptomicsMast cell expression data from murine aryl hydrocarbon receptor (AhR) null, AhR_d and normal control mice
We found that AhR, a unique chemical sensor, is critical in controlling mast cell differentiation, growth and function in vitro and in vivo. This study seeks to further the understanding of the mechanisms of how AhR control mast cell homeostasis. Three different batches of bone marrow derived mast c...
ORGANISM(S): Mus musculus 
2013-04-01 | E-GEOD-44715 | biostudies-arrayexpress
TranscriptomicsTet2-/-Flt3ITD and WT stem and progenitor cells
The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comp...
ORGANISM(S): Mus musculus 
2015-05-01 | E-GEOD-57244 | biostudies-arrayexpress
GenomicsHydroxymethylation at gene regulatory regions directs stem cell commitment during erythropoiesis
CD34 positive hematopoietic stem cells were differentiated into erythroid lineage. Next generation sequencing (NGS) of 5hmC affinity pulldown and RNAseq were performed in four time point of different stages of erythroid differentiation. 4 RNA-Seq Samples (d0, d3, d7 and d10); 4 affinity-pulldown (d...
ORGANISM(S): Homo sapiens 
2014-03-28 | E-GEOD-40243 | biostudies-arrayexpress
GenomicsFLT3-ITD mutations and TET2 loss synergistically alter global DNA methylation in AML
Studies of AML patient samples have shown that specific combinations of AML disease alleles confer an adverse outcome, however, in vivo models do not exist for the majority of common, poor-prognosis genotypes. Here we show that TET2/FLT3 mutations can cooperate to induce AML in vivo using a genetic...
ORGANISM(S): Mus musculus 
2015-04-14 | E-GEOD-57114 | biostudies-arrayexpress
TranscriptomicsTet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation
Recurrent somatic mutations in TET2 and in other genes that regulate the epigenetic state have been identified in patients with myeloid malignancies and in other cancers. However, the in vivo effects of Tet2 loss have not been delineated. We report here that Tet2 loss leads to increased stem-cell...
ORGANISM(S): Mus musculus 
2011-07-01 | E-GEOD-27816 | biostudies-arrayexpress
UnknownA prognostic model for use before elective surgery to estimate the risk of postoperative pulmonary complications (GSU-Pulmonary Score): a development and validation study in three international cohorts.
Not available
S-EPMC12056793 | biostudies-literature
UnknownAssessing the genetic association between vitamin B6 metabolism and genetic generalized epilepsy.
Not available
S-EPMC6796782 | biostudies-literature
UnknownSARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study.
Not available
S-EPMC7995808 | biostudies-literature
Sort   by:  
 Page size