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ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-WIPI2d
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043701 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-Rubicon
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043695 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
ProteomicsTMT-based proteomic analysis of Atg5-/- mouse brain
The brains of neonatal mice (Atg5+/+ in triplicate, Atg5-/- in quadruplicate, and Atg5-/-;NSE-Atg5 in triplicate) were lysed in 500 µL of 6 M guanidine-HCl containing 100 mM Tris-HCl, pH8.0, and 2 mM DTT. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 g fo...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-01 | PXD049220 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without LLOMe for 1 h or 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD043288 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen treated with MK6-83
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without MK6-83 for 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed with ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD053327 | JPOST Repository
ProteomicsTMTpro-18plex analysis of siSTK38 or siLuc HeLa cells treated with EBSS or LLOMe
HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
2023-11-21 | PXD039312 | JPOST Repository
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