Sort   by:  
 Page size 
ProteomicsProximity biotinylation of NCI-H226 cells with gefitinib alone treatment by EGFR-FabID
NCI-H226 cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identifi...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043527 | JPOST Repository
ProteomicsProximity biotinylation of A431 cells with gefitinib alone treatment by EGFR-FabID
A431cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043526 | JPOST Repository
ProteomicsProximity biotinylation of NCI-H226 cells by EGFR-FabID
NCI-H226 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were ...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039451 | JPOST Repository
ProteomicsProximity biotinylation of A431 cells by EGFR-FabID
A431 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were puri...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039450 | JPOST Repository
ProteomicsProximity biotinylation of Expi293F cells stably expressing EGFR by EGFR-FabID
Expi293F cells stably expressing EGFR were treated with AGIA-FabID or EGFR-FabID in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043525 | JPOST Repository
ProteomicsProximity biotinylation of Expi293F cells overexpressing EGFR by EGFR-FabID
Expi293F cells were transfected with pcDNA3.1-EGFR and treated with AGIA-FabID or EGFR-FabID in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS a...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039449 | JPOST Repository
ProteomicsMS analysis of BCG and recombinant BCG "70C-RBD"
BCG and recombinant BCG "70C-RBD" were cultured in Sauton media. Culture filtrate and cells lysate were separated by SDS-PAGE. The bands at 40kDa and 60kDa were excised and proteins were reduced and alkylated. In-gel digestion (Trypsin) was performed. The samples were analyzed by TripleTOF5600+ conj...
ORGANISM(S): Cellular Organisms 
2023-12-07 | PXD046929 | JPOST Repository
Sort   by:  
 Page size