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TranscriptomicsRIP-seq identification of RNAs enriched on RNA polymerase and the primary sigma factor (sigma A) in Mycobacterium tuberculosis
6S RNA is a small RNA with specific secondary structure that associates with the complex of RNA polymerase (RNAP) and the primary sigma factor in majority of bacteria. In Mycobacterium smegmatis, Ms1 interacts with the RNAP core without the sigma factor and probably replaces 6S RNA. It is currently ...
ORGANISM(S): Mycobacterium tuberculosis 
2024-02-09 | E-MTAB-12350 | biostudies-arrayexpress
GenomicsChIP-seq identification of CrsL (MSMEG_5890) binding sites in Mycobacterium smegmatis
CsrL (MSMEG_5890) associates with CarD and RNA polymerase in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strain with CrsL (LK3051), where an additional copy of CrsL under anhydrotetracycline inducible promoter was stably inserted into M. smegmatis genome. CrsL-FLAG was expressed in e...
ORGANISM(S): Mycolicibacterium smegmatis MC2 155 
2025-11-01 | E-MTAB-12348 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Mycobacterium smegmatis mc2 155 negative control strain (NC), carD (MSMEG_6077) and crsL (MSMEG_5890) knockdown strains, from exponential and stationary phase cells.
CarD is an essential transcription factor for Mycobacterium smegmatis. We identified a new interaction partner of CarD and named it CrsL (MSMEG_5890). To address the genome-wide effect of CrsL, we generated knockdown strains for both carD (MSMEG_6077) and crsL (MSMEG_5890) genes in Mycobacterium sme...
ORGANISM(S): Mycolicibacterium smegmatis 
2025-11-01 | E-MTAB-13812 | biostudies-arrayexpress
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