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Effect of temperature variation on zebra fish health and behaviour Water temperature is an important environmental parameter that influences the distribution and the health of fishes. Moreover, temperature has a central role in ectothermic animals affecting their physiology and behaviour. The aim of...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-01-26 | PXD009934 | Pride
This dataset contains single-cell RNA sequencing (scRNA-seq) profiles of zebrafish venous and lymphatic endothelial cells across development. Endothelial cells were isolated by FACS from transgenic zebrafish embryos and larvae at 36 hpf, 48 hpf, 5 dpf (120 hpf), and 7 dpf (168 hpf) using endothelial...
ORGANISM(S): Danio rerio 
We describe a method for isolating RNA suitable for high-throughput RNA sequencing (RNA-seq) from small numbers of fluorescently labeled cells isolated from live zebrafish (Danio rerio) embryos without using costly, commercially available columns. This method ensures high cell viability after dissoc...
ORGANISM(S): Danio rerio 
The physical connection between mitochondria and endoplasmic or sarcoplasmic reticulum is an essential signaling hub to ensure organelle and cellular functions. In skeletal muscle, ER/SR-mitochondria calcium (Ca2+) signaling is crucial to maintain cellular homeostasis during physical activity. High ...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-07-25 | PXD044765 | Pride
To monitor the relative regulatory contributions of RNA production, processing and degradation, we sampled RNA from mouse DCs every 15 minutes, for the first 3 hours of their response to LPS (13 samples in total), following a short (10 minute) metabolic labeling pulse with 4sU preceding the sampled ...
ORGANISM(S): Mus musculus 
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