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ProteomicsMudPIT LiPMS analyses of whole cell protein extracts from Saccharomyces cerevisiae grown at 21C and 35C
Sample Preparation. Three biological replicates of wild type yeast cells (BY4741) were cultured with YEPD either at 21C or 35C overnight (ca. 18hrs) to OD600 around 2. Pre-warmed/chilled fresh YEPD was next used to dilute the cultures, which were grown for an additional 6hrs to OD600 around 1. 120m...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2021-02-01 | MSV000086790 | MassIVE
ProteomicsMudPIT analyses of the proteins co-purified with wild type-Hzg-3XHA/Flag and phosphatase dead-Hzg-3XHA/Flag affinity purifications from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell expressing Hzg albeit low level, lines expressing C-terminally 3XHA-Flag-tagged wild-type and phosphatase dead (PD) Hzg were generated. A parental cell line was used as a negative control. Nuclear and cytoplasmic extracts from the Hzg-WT, Hzg-mutant and...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2019-01-08 | MSV000083305 | MassIVE
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