Sort   by:  
 Page size 
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsProteomic Sample Preparation in the Low Microgram Range – A Critical Evaluation of Current Workflows
Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Over the past years, several protocols been specifically developed for the preparation of samples, which are limited in quantity. In the present study, we carried ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-05-11 | PXD006760 | Pride
Single-pot solid-phase-enhanced sample preparation (sp3) Filter-aided sample preparation (fasp) In-stagetip digestion (ist) Sample preparation Bottom-up proteomics 
ProteomicsSP3 protocol for proteomic plant sample preparation prior LC-MS/MS
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-26 | PXD022688 | Pride
Protein cleanup Arabidopsis thaliana Bottom-up Mass spectrometry Sds removal Sp3 Fasp Carboxylated magnetic beads 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Sus scrofa domesticus (domestic pig) 
2020-03-27 | PXD015840 | Pride
Fresh-frozen tissue Proteomics Sp3 Cells Sample preparation 
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Homo sapiens (Human) 
2022-08-04 | PXD028736 | Pride
Proteomics Solvent precipitation Sample preparation 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) 
2020-01-10 | PXD014556 | Pride
Sp3 Clinical proteomics Pulmonary adenocarcinoma Sample preparation Low-input High-throughput Automation 
ProteomicsComparison of In-Gel and SP3 Mass Spectrometry Sample Preparation Protocols
Sensitivity, robustness, and reproducibility of sample preparation are main determinants of data quality in bottom-up mass spectrometry-based proteomics. To this end, in-gel protein clean-up and digestion has been used for decades and is characterized by its robustness and compatibility with harsh l...
ORGANISM(S): Homo sapiens (Human) 
2026-08-24 | PXD064954 | Pride
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-03 | PXD028768 | Pride
Microparticles Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation Sample preparation 
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture protein aggregates, ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2022-08-01 | PXD032095 | Pride
Microparticles Proteomics Sample preparation Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation 
ProteomicsImpact of Sample Preparation Strategies on The Quantitative Accuracy of Low-Abundance Serum Proteins in Shotgun Proteomics
Serum proteomics plays a crucial role in biomarker discovery and disease research, yet the selection of an optimal sample preparation method remains challenging. Evaluating the accuracy of protein quantitation is of major importance and a vital part of a benchmarking study in proteomics, since clini...
ORGANISM(S): Homo sapiens (Human) 
2025-09-15 | PXD062785 | Pride
Reproducibility Quantitative accuracy Serum proteomics Depth of coverage 
Sort   by:  
 Page size