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ProteomicsReproducibility of label-free single-shot phosphoproteomics applied to CRC cell lines
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080715 | MassIVE
ProteomicsReproducibility of label-free single-shot phosphoproteomics applied to CRC cell lines
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo sapiens (Human) 
2015-04-15 | PXD001546 | Pride
Human Single-shot Crc Label-free Phosphoproteomics Colorectal cancer 
ProteomicsSingle shot CZE-MS/MS for phosphoproteomics
The dependence of capillary zone electrophoresis separations on the charge state of analyte is useful for the analysis of many post-translational modifications in proteins. In this work, we coupled CZE to an Orbitrap Fusion Lumos Tribrid platform with advanced peak determination algorithm for phosph...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-13 | PXD012888 | Pride
Cze Phosphoproteomics Orbitrap fusion lumos tribrid Advanced peak determination algorithm 
ProteomicsSimple, single-shot phosphoproteomic analysis of heat-stable tau
We developed a simple, antibody-free approach for single shot analysis of tau phosphorylation across the entire protein by liquid-chromatography tandem mass spectrometry (LC-MS/MS) to study age-related changes in tau phosphorylation. This methodology is species independent; thus, while initially dev...
ORGANISM(S): Rattus norvegicus (Rat) Macaca mulatta (Rhesus macaque) 
2021-11-25 | PXD027971 | Pride
Phosphoproteomics Brain Tau Heat-stable 
GenomicsRapid protection induced by a single-shot Lassa vaccine in cynomolgus monkeys
Rapid protection induced by a single-shot Lassa vaccine in cynomolgus monkeys
PRJNA934837 | ENA
ProteomicsDeep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS
This study demonstrates how the latest UHPLC (ultra-high-performance liquid chromatography) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with nano-flow liquid c...
ORGANISM(S): Homo sapiens (Human) 
2022-09-12 | PXD035665 | Pride
Human Lcms Cells Orbitrap 
ProteomicsSingle-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as an invaluable platform for top-down proteomics. However, the scale of top-down proteomics from CZE-MS/MS is still limited due to the low loading capacity and narrow separation windo...
ORGANISM(S): Escherichia coli 
2017-10-30 | PXD007273 | Pride
Top-down proteomics Cze-ms/ms E.coli 
ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
UnknownSingle-shot compressed optical field topography.
Not available
S-EPMC9343635 | biostudies-literature
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