Sort   by:  
 Page size 
TranscriptomicsRIP-Chip of endogenous Staufen and GFP-tagged Staufen from 0-3 hour Drosophila embryos
Staufen-associated mRNAs were identified in early Drosophila embryos by RNA-co-immunoprecipitation followed by microarray analysis (RIP-Chip), using two complementary approaches: RIP-Chip from wild-type embryos using a synthetic anti-Staufen antibody, and RIP-Chip from transgenic GFP-Staufen-express...
ORGANISM(S): Drosophila melanogaster 
2013-09-05 | E-GEOD-43418 | biostudies-arrayexpress
TranscriptomicsSMAUG Is a Major Regulator of Maternal mRNA Destabilization in Drosophila
In animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserve...
ORGANISM(S): Drosophila melanogaster 
2007-09-13 | E-GEOD-8910 | biostudies-arrayexpress
TranscriptomicsRIP-Chip of BRAT and PUM from 0-3 hour Drosophila melanogaster embryos
BRAT-associated mRNAs and PUM-associated mRNAs were identified in early Drosophila embryos by RNA co-immunoprecipitation of the endogenous proteins using synthetic antibodies, followed by microarray analysis (RIP-Chip). Nine RNA co-immunoprecipitations were performed. This includes 3 biological repl...
ORGANISM(S): Drosophila melanogaster 
2015-03-28 | E-GEOD-60466 | biostudies-arrayexpress
TranscriptomicsGlobal regulation of mRNA translation and stability in the early Drosophila embryo by the Smaug RNA-binding protein- Polysome gradient/microarray study
To gain a panoramic view of Smaug function in the early embryo we identified mRNAs that are bound to Smaug using RNA co-immunoprecipitation followed by hybridization to DNA microarrays. We also identified the mRNAs that are translationally repressed by Smaug using polysome gradients and microarrays....
ORGANISM(S): Drosophila melanogaster 
2013-12-03 | E-GEOD-50026 | biostudies-arrayexpress
TranscriptomicsGenome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition
Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete. In the RNAcompete assay, purified GST-tagged BRAT is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agil...
ORGANISM(S): synthetic construct 
2015-03-30 | E-GEOD-60498 | biostudies-arrayexpress
TranscriptomicsIdentification of Smaug target mRNAs in the early Drosophila embryo using RIP-Chip
To identify SmaugM-bM-^@M-^Ys target mRNAs on a genome-wide scale we used ribonucleoprotein (RNP) co-immunoprecipitation followed by microarray analysis of the co-purifying mRNAs (RIP-Chip). Extracts, prepared from wild-type embryos collected 0-3 hours post-egglaying, were immunoprecipitated with an...
ORGANISM(S): Drosophila melanogaster 
2013-12-03 | E-GEOD-49943 | biostudies-arrayexpress
Sort   by:  
 Page size