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TranscriptomicsATAC-seq of gata5GFP+/- cells in WT and Gata5/6 deficient zebrafish embryos during gastrulation
Gata5/6 are essential regulators in zebrafish heart development. To understand how Gata5/6 affects the chromatin accessibility and regulates cardiac gene expression, we FACS-isolated GFP+ and GFP- cells from Tg(gata5:GFP) transgenic embryos in both control and Gata5/6 deficient embryos (injected wit...
ORGANISM(S): Danio rerio 
2021-12-31 | E-MTAB-10427 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptome analysis of gata5GFP labelled WT and Gata5/6 deficient cells from early gastrulation to early somitogenesis in zebrafish
TgBAC(gata5:EGFP) transgenic line was used to isolate mesendoderm cells enriched for cardiac lineage. Wild type embryos at 6, 8, 10, 13 hours post fertilization (hpf) and morpholinos injected Gata5/6 know-down (KD) embryos at 13 hpf were pre-screened for GFP signal and dissociated into single-cell s...
ORGANISM(S): Danio rerio 
2021-12-01 | E-MTAB-9193 | biostudies-arrayexpress
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