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Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG. Set of arrays organized by shared biological co...
ORGANISM(S): Homo sapiens 
Five ITP and five control whole blood samples were collected with IRB approval. The age range of ITP patients was 1-54 years, and the range in platelet counts was 2,000-98,000 W 109/L. We examined only one ITP sample for antiplatelet antibodies, and found it to be positive. Total RNA was isolated fr...
ORGANISM(S): Homo sapiens 
Foreskin fibroblasts CRL 2091 (ATCC) were serum starved for 48 hours, and harvested at the indicated time points after switching to media with 10% FBS essentially as described (Iyer et al., 1999). RNA from all of the sampled time points were pooled as reference RNA to compare with RNA from individua...
ORGANISM(S): Homo sapiens 
Five ITP and five control whole blood samples were collected with IRB approval. The age range of ITP patients was 1-54 years, and the range in platelet counts was 2,000-98,000 W 109/L. We examined only one ITP sample for antiplatelet antibodies, and found it to be positive. Total RNA was isolated fr...
ORGANISM(S): Homo sapiens 
Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG.
ORGANISM(S): Homo sapiens 
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