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AGS cell line was cultured in standard T25 flasks (2D condition) and in T25 flasks precoated with 50 M-5L/cm2 of matrigel basement membrane (BD Biosciences) at a 1.5:1 proportion to RPMI serum-free medium (3D condition, overlay method). Cultures were maintained for 14 days with medium change every t...
ORGANISM(S): Homo sapiens 
To evaluate the gene expression profiling of peripheral leukocytes in different outcomes of SARS-CoV-2 infections, whole blood samples were collected from individuals with positive SARS-CoV-2 nasopharyngeal swab by RT-PCR (54 patients) and healthy uninfected individuals (12 volunteers). Infected pat...
ORGANISM(S): Homo sapiens 
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