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TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC) 8624 double deletions of kdpe and cra grown in DMEM
Analysis of effect of deletion of the two transcriptional factors KdpE and Cra on gene expression of EHEC 8624 A double mutant of kdpE and cra in the EHEC 8624 was grown to OD600=1.0 in DMEM then processed according to manufacturer's specifications: http//www.affymetrix.com/support/technical/manual...
ORGANISM(S): Escherichia coli 8624 
2013-07-03 | E-GEOD-43659 | biostudies-arrayexpress
TranscriptomicsThe role of qseE, qseF and qseG in the regulation of EHEC virulence
Escherichia coli 8624 and the isogenic mutants in qseE, qseF and qseG are compared to determine the role that each of the genes play in regulation of the transcriptome. These results are verified by qRT-PCR and reveal the important role of this three-component signaling system. Escherichia coli 862...
ORGANISM(S): Escherichia coli 
2010-04-05 | E-GEOD-12831 | biostudies-arrayexpress
TranscriptomicsQseA regulation of virulence factors in EHEC
Enterohemorrhagic E. coli (EHEC) colonizes the large intestine and causes attaching and effacing lesions (AE). Most of the genes involved in the formation of AE lesions are encoded within a chromosomal pathogenicity island termed the Locus of Enterocyte Effacement (LEE). The LysR-like transcriptiona...
ORGANISM(S): Escherichia coli 
2010-09-07 | E-GEOD-18118 | biostudies-arrayexpress
TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC)8624 double deletions of qseC and qseE grown in DMEM
Analysis of effect of deletion of the two adrenergic kinases QseC and QseE on gene expression of EHEC 8624 in the absence and presence of epinephrine A double mutant of qseC and qseE in the EHEC 8624 was grown in the absence or presence of epinephrine to OD600=1.0 in DMEM then processed according to...
ORGANISM(S): Escherichia coli O157:H7 
2012-03-15 | E-GEOD-33895 | biostudies-arrayexpress
TranscriptomicsEHEC and K-12 E. coli QseD mutant analysis
We used arrays to examine the overall transcriptional differences between WT K-12 E. coli, and EHEC 86-24 and their corresponding QseD (yjiE) mutants. Strains were grown to OD600 ~1.0, RNA was harvested, cDNA synthesized, fragmented, labeled and hybridized to the chips. Expression of the QseD mutan...
ORGANISM(S): Escherichia coli 
2010-03-07 | E-GEOD-20413 | biostudies-arrayexpress
TranscriptomicsEffects of sidA and AHL on EHEC virulence
A study on the effects of an sdiA mutant and the AHL molecule on the virulence of EHEC Comparison of wild type E. coli 8624 with and without AHL to and E. coli 8624 sdiA mutant with and without AHL to determine the effects of each factor on gene expression
ORGANISM(S): Escherichia coli 
2010-04-09 | E-GEOD-13562 | biostudies-arrayexpress
TranscriptomicsExpression data from an Avian pathogenic Escherichia coli strain
Avian pathogenic Escherichia coli strains frequently cause extra-intestinal infections and are responsible for significant economic losses in the poultry industry worldwide. APEC isolates are closely related to human extraintestinal pathogenic E.coli strains and may also act as pathogens for humans....
ORGANISM(S): Escherichia coli 
2010-09-09 | E-GEOD-21893 | biostudies-arrayexpress
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