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Mature males and virgin queens from Atta colombica ants were collected in panama, and their seminal fluid (SF) and spermathecal fluid (SpF), respectively, were extracted and analysed by shot-gun proteomics. For this, using a pooled SF sample of 25 males from five colonies, squeezing their abdomens t...
ORGANISM(S): Atta columbica 
2019-01-03 | PXD011306 | Pride
Amphioctopus fangsiao is an economically important cephalopod species in northern China. A notable reproductive adaptation of this species is the prolonged storage of sperm in the female spermatheca for up to 8 months post-mating, until oocyte maturation and release. Spermathecal fluid, as a critica...
ORGANISM(S): Amphioctopus Fangsiao 
2025-11-20 | PXD071105 |
Polyandrous ant queens are inseminated early in their life and store sperm mixtures for a potential reproductive life of decades. However, they cannot re-mate later in life and are thus expected to control the loss of viable sperm because their life-time reproductive success is ultimately limited by...
ORGANISM(S): Atta columbica 
2019-01-03 | PXD011320 | Pride
To reveal the molecular basis of the long-term sperm storage mechanisms in ant queens, protein profiles enriched in the spermathecal fluid relative to the hemolymph were identified in Lasius japonicus using data-independent acquisition (DIA)-based quantitative proteomics technology.
ORGANISM(S): Cellular Organisms 
We sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the spermathecal fluid (sperm removed). Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buff...
ORGANISM(S): Apis Mellifera 
2020-03-04 | MSV000085056 | MassIVE
single nucleus RNA-seq study profiling the transcriptional response of Aedes aegypti spermathecal secretory cells to mating and injection with male accessory glad lysate
Spermathecal fluid was extracted from adult queens at two different life stages (virgins and mated 2 week old) and separated from sperm by centrifugation. Queens were all sourced from the same grafting event and mated on the same schedule. Virgin queens were 2 days old at sampling. Some mated 2 week...
ORGANISM(S): Apis Mellifera (ncbitaxon:7460) 
2024-10-23 | MSV000096180 | MassIVE
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