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Despite the general acceptance of formic acid as the additive of choice for peptide reversed-phase LC-MS/MS applications, some still argue that the selection of acetic acid represents a better option. To settle this debate, we investigated both the difference in MS sensitivity and chromatographic be...
ORGANISM(S): Homo Sapiens (human) 
Tryptic digested E. coli dataset fractionated by SCX stagetip and applied to test the CCS prediction model. The Peaklist from the MS/MS spectra as mascot generic format (MGF) was created by compass DataAnalysis 5.2.216.271.5315. Protein and peptides were identified by by Mascot-Percolator.
ORGANISM(S): Escherichia Coli 
Tryptic digested E. coli dataset fractionated by SCX stagetip and applied to test the CCS prediction model.
ORGANISM(S): Escherichia Coli 
The contribution of peptide amino-acid sequence to collision cross-section values (CCS) has been investigated using a dataset of ~134,000 peptides of four different charge states (1+ to 4+). The migration data collection was acquired using a two-dimensional LC/trapped ion mobility spectrometry/quadr...
ORGANISM(S): Homo Sapiens (human) 
We used 2D-LC-MS/MS methods to examine the impact of the biologically important PTM citrullination and its analogue carbamylation on peptide retention. Three first-dimension separation systems were evaluated: high-pH, HILIC and SCX, with the instrument interface separation happening at low-pH RP for...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Sus Scrofa (ncbitaxon:9823) Gallus Gallus (ncbitaxon:9031) Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
To capture interplay between biological pathways we analyzed the proteome from matched lung tissue and bronchoalveolar lavage fluid (BALF) of individual allergen-naive and house dust mite (HDM)-challenged BALB/c mice, a model of allergic asthma. Unbiased label-free LC-MS/MS analysis quantified 2,675...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-08-24 | MSV000086003 | MassIVE
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