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ProteomicsCombining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification )
Data Independent Acquisition (DIA) is increasingly preferred over Data Dependent Acquisition (DDA) due to its higher throughput and fewer missing values. Whereas DDA often utilizes stable isotope labeling to improve quantification, DIA mostly relies on label-free approaches. Efforts to integrate DIA...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-10-14 | PXD052080 | Pride
Multiplexing Dia Plexdia Silac Proteomics Benchmark 
ProteomicsComparative assessment of quantification methods for tumor tissue phosphoproteomics
The choice of quantification method is critical for study design and comparison of cancer phosphoproteomes. We comparatively assessed label-free quantification (LFQ), spike-in-SILAC (stable isotope labeling of amino acids in cell culture) and tandem mass tag (TMT) labeling techniques for quantitativ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-09 | PXD030450 | Pride
Phosphoproteomics Tissue Ovarian cancer Quantification 
UnknownCombining Data Independent Acquisition With Spike-In SILAC (DIA-SiS) Improves Proteome Coverage and Quantification.
Not available
S-EPMC11795695 | biostudies-literature
UnknownSILAC-based quantitative MS approach for real-time recording protein-mediated cell-cell interactions.
Not available
S-EPMC5981645 | biostudies-literature
ProteomicsMouse aging oocyte proteome comparison
Here we asked if oocyte proteomes are representative of the transcriptomes, how the abundance of specific genes’ mRNA and protein responds to maternal aging, and if oocyte aging presents the features characteristic of somatic aging. To address these questions on the proteomic level, we employed stab...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-14 | PXD000512 | Pride
Mus musculus Embryonal carcinoma cells Spike-in Silac Oocyte 
ProteomicsSpike-in SILAC MS of C. elegans CPEB homolog mutants.
Comparative proteomic study between C. elegans wild-type N2 and CPEB homolog mutants: fog-1, cpb-2, and cpb-3.
ORGANISM(S): Caenorhabditis elegans 
2017-08-02 | PXD004104 | Pride
Hilic C. elegans Cpeb proteins Maxquant Spike-in silac Lc-ms/ms 
ProteomicsSILAC-based quantitative proteomics of endoplasmic reticulum stress
ER stress and the unfolded protein response (UPR) involve extensive proteome remodeling in many cell compartments. However, a comprehensive analysis has been lagging due to technological limitations. Here we employ SILAC-based proteomics to quantify over 6,200 proteins at increasing concentrations o...
ORGANISM(S): Homo sapiens (Human) 
2019-11-01 | PXD013496 | Pride
Unfolded protein response Proteomics Silac Tunicamycin Endoplasmic reticulum stress 
ProteomicsDIA Spike-in SILAC Revisions; additional submission including single-cell like amounts
Additional data and analyses for submission of DIA-SiS
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-10-14 | PXD054343 | Pride
Silac dia benchmark proteomics 
UnknownIdentification of disease specific pathways using in vivo SILAC proteomics in dystrophin deficient mdx mouse.
Not available
S-EPMC3650321 | biostudies-literature
ProteomicsProteomic Evaluation of PANDER TG Mice
Previous studies have demonstrated elevated circulating levels of PANcreatic DERived factor (PANDER) overexpression induced a selective hepatic insulin resistant phenotype in the liver of the PANDER transgenic mouse model. Here we utilized SILAC for "spike-in" as a quantitative mass spectrometry bas...
ORGANISM(S): Mus musculus (Mouse) 
2016-09-29 | PXD004171 | Pride
Fam3b Silac Pander Liver 
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