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Transcriptomicsarray-CGH on single blastomeres from day-4 human preimplantation fresh and frozen embryos
In total, 240 single blastomeres from nine top-quality day-4 embryos frozen at day 3 of development and four fresh top-quality day-4 embryos that had one-cell biopsy on day 3 for preimplantation genetic diagnosis (PGD) were collected. Blastomeres' DNA was amplified using SurePlex DNA Amplification S...
ORGANISM(S): Homo sapiens 
2013-04-05 | E-GEOD-39518 | biostudies-arrayexpress
Transcriptomicsarray-CGH on single blastomeres from day-3 human cleavage stage embryos
Seventy blastomeres from fourteen frozen-thawed supernumerary human preimplantation embryos were disassociated and genomic was amplified using Multiple Displacement Amplification. BAC array-CGH was performed on the amplified products. BAC array-CGH on single blastomeres amplified by Multiple Displac...
ORGANISM(S): Homo sapiens 
2012-10-15 | E-GEOD-34290 | biostudies-arrayexpress
TranscriptomicsArray-based comparative genomic hybridization study of human embryonic stem cell lines
G-banding of human embryonic stem cells (hESC) has proved their predisposition to aneuploidy of chromosomes 12, 17 and X. Now, using array-based comparative genomic hybridization, we find that hESC also accumulate other recurrent chromosomal abnormalities, such as duplications of stemness genes, sub...
ORGANISM(S): Homo sapiens 
2010-05-17 | E-GEOD-13422 | biostudies-arrayexpress
TranscriptomicsNon-clonal mosaicism in human somatic and embryonic stem cells revealed by single-cell array-based copy-number variation analysis
Although it is known that cultured human cells acquire copy number variations over time, little is known about the mutation frequency in individual cells. Here we describe that human somatic and embryonic stem cell cultures show significant fractions of cells carrying unique chromosomal abnormalitie...
ORGANISM(S): Homo sapiens 
2014-06-24 | E-GEOD-51126 | biostudies-arrayexpress
TranscriptomicsSingle-cell aneuploidy detection by array CGH
In this study, we extend array CGH technology by making the accurate detection of chromosomal imbalances possible from a single fibroblast and blastomere following Phi29 DNA polymerase amplification. Firstly, array CGH experiments were performed on four different fibroblast cell lines, derived from ...
ORGANISM(S): Homo sapiens 
2010-07-01 | E-GEOD-3642 | biostudies-arrayexpress
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