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TranscriptomicsKey roles for SRF in embryonic epidermal differentiation
During development, a polarized sheet of epidermal cells undergoes stratification and differentiation to produce the skin barrier. Through mechanisms poorly understood, the process involves adhesion and Notch signaling. To elucidate how epidermal embryogenesis is governed, we conditionally targeted ...
ORGANISM(S): Mus musculus 
2011-01-20 | E-GEOD-25548 | biostudies-arrayexpress
TranscriptomicsMx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells
Analysis of hematopoietic LSK(Lin-Sca1+c-Kit+) cells lacking the Serum response factor (SRF) gene. Results provide insight into the role of SRF in regulating genetic programs important for hematopoietic stem cell development Comparison of the gene expression profile between murine Lin-Sca1+c-Kit+ ce...
ORGANISM(S): Mus musculus 
2010-08-12 | E-GEOD-23556 | biostudies-arrayexpress
TranscriptomicsMRTF-SRF signaling is required for seeding of HSC/Ps in bone marrow during development
Chemokine signaling is important for the seeding of different sites by hematopoietic stem cells during development. Serum Response Factor (SRF) controls multiple genes governing adhesion and migration, mainly by recruiting members of the Myocardin-Related Transcription Factor (MRTF) family of G-acti...
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-63820 | biostudies-arrayexpress
TranscriptomicsSRF Overexpression Leads to Altered Expression of MicroRNAs and Contributes to Cardiac Hypertrophy
The role of SRF in the regulation of microRNA expression and microRNA biogenesis in cardiac hypertrophy has not been well established. In this report, we employed a distinct transgenic mouse model to study the impact of SRF on cardiac microRNA expression and microRNA biogenesis. Cardiac-specific ove...
ORGANISM(S): Mus musculus 
2011-02-16 | E-GEOD-23044 | biostudies-arrayexpress
TranscriptomicsSmall RNA sequencing in mouse cardiomyocytes after siRNA mediated knockdown of Srf
We performed two independent siRNA mediated knockdowns of Srf (Srf si1 & Srf si2) and an unspecific siRNA (siNon) in mouse cardiomyocytes HL-1 cells. Small RNAs were sequenced by Illumina/Solexa next-generation (single-end) sequencing technology. The sequence reads were mapped to the mouse reference...
ORGANISM(S): Mus musculus 
2011-01-03 | E-GEOD-19066 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic stem (ES) cells overexpressing serum response factor SRF to identify potential transcriptional targets of SRF
Empty vector, SRF-dM-VP16 (control construct lacking the SRF DNA binding domain), or SRF-VP16 were expressed in wild-type ES cells (E14wt), SRF heterozygotes (E99-/+) or two SRF ko cell lines (E81-/-,E100-/-). RNA was harvested 72h after transfection. Each condition was performed in duplicate, excep...
ORGANISM(S): Mus musculus 
2007-11-06 | E-GEOD-1948 | biostudies-arrayexpress
GenomicsIdentification of transcription factor Srf binding sites and histone 3 actetylated regions in mouse cardiomyocytes
We investigated the cardiac transcription network driven by the DNA-binding key factor Srf in combination with epigenetic marks of histone 3 acetylation (H3ac). Srf has been shown to play a key role in cardiac cell growth and muscle gene regulation. However, we still have limited understanding of th...
ORGANISM(S): Mus musculus 
2011-01-03 | E-GEOD-19065 | biostudies-arrayexpress
TranscriptomicsHeart specific moderate over-expression of serum response factor (SRF) in 6mo mouse.
To identify in vivo new cardiac SRF target genes and to study the response of these novel genes to SRF overexpression, we employed a cardiac-specific, transgenic mouse model that has a phenotype in young adulthood which resembles that of the typically aged heart. Using this “cardiac aging” model, we...
ORGANISM(S): Mus musculus 
2011-08-02 | E-GEOD-19874 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic stem (ES) cells following stimulation with fetal bovine serum at various time points up to 180 minutes to identify potential targets of SRF
Murine ES cells of different Srf genotype (E14wt, E100-/-, E100-/- rescue (Srf transgene)) were stimulated with FBS for 0-180 min. RNA was harvested and hybridized to Affymetrix MGU74A arrays.
ORGANISM(S): Mus musculus 
2007-11-02 | E-GEOD-1949 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to identify binding sites for Gata4, Mef2a, Nkx2.5, Srf, p300, Pol_II, H3ac, H3K4me1 in mouse cardiomyocyte cell line, HL1
The aim of the experiment was to identify genome wide binding sites for Gata4, Mef2a, Nkx2.5, Srf, p300, Pol_II, H3ac, H3K4me1 by using Chromatin Immunoprecipitation followed by microarray analysis (ChIP-chip) in HL1 cells.
ORGANISM(S): Mus musculus 
2011-01-05 | E-TABM-378 | biostudies-arrayexpress
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