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TranscriptomicsPurification of TAP::ALG-1 complexes and associated RNA from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2) animals
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein We purified TAP::ALG-1 complexes from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2, serving as a mock control) animals...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32941 | biostudies-arrayexpress
TranscriptomicsExpression analysis of WS4303 (wild type) vs WS5041 (mir-58 mutants) total mRNA
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein (C. elegans strain WS4303). We crossed the TAP::ALG-1 transgene into the mir-58(n4640) mutant background to generate the strain WS5041...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32943 | biostudies-arrayexpress
TranscriptomicsIdentification of miRNA target genes in C. elegans by RIP-chip-SRM
RIP-chip-SRM : a New Combinatorial Large Scale Approach Identifies a Set of Translationally Regulated bantam/miR 58 Targets in C. elegans RNA binding protein immunopurification + microarray + targeted protein quantification via Selected Reaction Monitoring This SuperSeries is composed of the follow...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32944 | biostudies-arrayexpress
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UnknownA prognostic model for use before elective surgery to estimate the risk of postoperative pulmonary complications (GSU-Pulmonary Score): a development and validation study in three international cohorts.
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S-EPMC12056793 | biostudies-literature
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