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ProteomicsA Novel Approach for Generating Antigen Interference-Free Anti-Peptide Polyclonal Antibodies for Quantitative Measurements of Low-Abundance Protein Biomarkers Using Ultra-Sensitive Anti-Peptide Immunocapture LC-MS/MS Assays
Antigen interference from residual antigen peptides remains a persistent challenge in anti-peptide immunocapture liquid chromatography-tandem mass spectrometry (IC-LC-MS/MS) assays for quantifying low-abundance protein biomarkers. In this manuscript, we introduce a novel approach that utilizes carri...
ORGANISM(S): Homo sapiens (Human) 
2026-09-04 | PXD076272 | Pride
Stable isotopically labeled peptide antigen Anti-peptide immunocapture Protein biomarker Antigen interference Lc-ms/ms 
ProteomicsQuantitative Proteomics Reveals that the OGT Interactome is Remodeled in Response to Oxidative Stress
The dynamic modification of specific serine and threonine residues of intracellular proteins by O-linked N-acetyl-β-D-glucosamine (O-GlcNAc) mitigates injury and promotes cytoprotection in a variety of stress models. The O-GlcNAc transferase (OGT) and the O-GlcNAcase (OGA) are the sole enzymes that ...
ORGANISM(S): Mus Musculus 
PXD021091 | panorama
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