The gut microbiome has been implicated in multiple human chronic gastrointestinal (GI) disorders. Determining its mechanistic role in disease has been difficult due to apparent disconnects between animal and human studies and lack of an integrated multi-omics view of disease-specific physiologica...
After co-transfection with with NOS1 and either wild-type or C251S PTBP1, SNO-RAC was performed to capture S-nitrosylated (SNO-) proteins, and mass spectrometry was used to identify these SNO-proteins.
Interacting proteins were co-immunoprecipitated with either wild-type or C251S PTBP1 after co-transfection with NOS1 for 48 hours. Mass spectrometry was used to identify interacting proteins.
