Yeast protein microarrays were utilized to investigate determinants of S-nitrosylation by biologically relevant low-mass S-nitrosothiols (SNOs). Large numbers of S-nitrosylated yeast proteins were identified after treatment with SNOs, among which those with active-site Cys thiols residing at N termi...
After co-transfection with with NOS1 and either wild-type or C251S PTBP1, SNO-RAC was performed to capture S-nitrosylated (SNO-) proteins, and mass spectrometry was used to identify these SNO-proteins.
Interacting proteins were co-immunoprecipitated with either wild-type or C251S PTBP1 after co-transfection with NOS1 for 48 hours. Mass spectrometry was used to identify interacting proteins.
