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ProteomicsIdentification of proteins stably associated with G9a/GLP (EHMT2/EHMT1) complex of histone methyltransferases
G9a (EHMT2) and the G9a-like protein GLP (EHMT1) form a stable G9a/GLP heterodimer in embryonic stem cells and function cooperatively to establish and maintain the abundant repressive H3K9me2 modification, in addition to modifying several non-histone proteins. The G9a-dependent H3K9me2 is implicated...
ORGANISM(S): Mus musculus (Mouse) 
2016-03-16 | PXD003466 | Pride
Shotgun proteomics G9a/glp (ehmt2/ehmt1) complex Embryonic stem cells Histones Mouse Chromatin 
GenomicsCell-autonomous function of LSH ATPase in de novo DNA methylation
Purpose: We aimed to determine whether the expression of either wild-type or catalytically inactive LSH, carrying a single point mutation in its ATP binding site (K237Q), could restore the levels and patterns of DNA methylation in Lsh-/- mouse embryonic fibroblasts (MEFs). Methods: Lsh-/- MEFs were...
ORGANISM(S): Mus musculus 
2016-07-21 | E-GEOD-64384 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human fibroblast cells transfected with a MDB-VP16 fusion to identify possible target genes for MBD1
We compared cells transfected with a MBD-VP16 fusion, with cells transfected with a control construct.
The purpose was to turn a repressor protein (MBD1) into an activator, and use differences in transcription to identify possible gene promoter targets.
A common reference was employed for all ...
ORGANISM(S): Homo sapiens 
2009-06-30 | E-MTAB-103 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of H3K9/K14 Acetylation and H3K27 trimethylation at promoters in the human lung embryonic fibroblast cell line MRC5
Genome-wide profiling of H3K9/K14 Acetylation and H3K27 trimethylation at promoters in the human lung embryonic fibroblast cell line MRC5
ORGANISM(S): Homo sapiens 
2013-11-11 | E-MTAB-2004 | biostudies-arrayexpress
GenomicsSmchd1 dependent and independent pathways determine developmental dynamics of CpG island methylation on the inactive X chromosome.
X chromosome inactivation involves multiple levels of chromatin modification, established progressively and in a stepwise manner during early development. The chromosomal protein Smchd1 has recently been shown to play an important role in DNA methylation of CpG islands (CGIs), a late step in the X i...
ORGANISM(S): Mus musculus 
2012-10-15 | E-GEOD-37333 | biostudies-arrayexpress
TranscriptomicsGenome-wide expression analysis in normal and immortalised and transformed human cell lines
Analysis of expression changes in the fetal human lung fibroblast cell line MRC5 over time (two time points: 50 and 100 population doublings) during immortalisation with the catalytic component of telomerase (hTERT) and in vitro transformation with SV40 Large T antigen and oncogenic H-RAS
ORGANISM(S): Homo sapiens 
2013-11-11 | E-MTAB-2003 | biostudies-arrayexpress
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