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TranscriptomicsGene expression profile in a breast cancer cell line upon hormonal treatments
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-alpha tagged with TAP-tag at the C-term (C-TAP-ER-alpha). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) fo...
ORGANISM(S): Homo sapiens 
2012-06-25 | E-MTAB-1196 | biostudies-arrayexpress
TranscriptomicsGene expression profile in breast cancer cell lines using miRNA over-expression
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag at the C-term (C-TAP-ER-beta). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) for ...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-MTAB-1493 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile in breast cancer cells
We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from MCF-7 cells expressing human ER-beta in absence of estrogen stimuli was subjected to size selection labrary preparation ond retrotranscribed. Obtained DNA was sequenced with the Illumina...
ORGANISM(S): Homo sapiens 
2014-11-13 | E-MTAB-2085 | biostudies-arrayexpress
Transcriptomicsendometrial_array_submission
Endometrial biopsies from tumor (T), adjacent normal (N) and hyperplastic (H) tissue specimens were obtained by histeroscopy from 5 patients
ORGANISM(S): Homo sapiens 
2015-04-01 | E-MTAB-2796 | biostudies-arrayexpress
Transcriptomicsendometrial_seq_submission
Endometrial biopsies from tumor (T), adjacent normal (N) and hyperplastic (H) tissue specimens (when available) were obtained by histeroscopy from 10 patients and 3 healty donor. RNA isolated from these samples was used to perform smallRNA-Seq by NGS and gene expression profiling by microarrays in ...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-MTAB-2795 | biostudies-arrayexpress
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