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We made an insulation (STITCH+30kb) allele of the MYC enhancer in human iPS cells. We integrated a transgene consisting of tetR-KRAB followed by DNA encoding the 2A peptide and the puromycin resistant gene with piggyBac transposition into the genome in the STITCH+30kb clone (STITCH/KRAB). We perform...
ORGANISM(S): Homo sapiens 
nChIP-seq for CTCF, H3K4me3, H3K27me3 and H3K9me3, in wild type (Hap), STITCH+30kb and STITCH/KRAB clones of human iPS cells.
We reconstituted arrays of CTCF binding sites (L1, L2, L3, L4, R1, R2, and R3) and devised a synthetic topological insulator with tetO for chromatin-engineering (STITCH). By coupling STITCH with tetR linked to the KRAB domain to induce heterochromatin and disable the insulation, we developed a drug-...
ORGANISM(S): Homo sapiens 
We reconstituted arrays of CTCF binding sites (L1, L2, L3, L4, R1, R2, and R3) and devised a synthetic topological insulator with tetO for chromatin-engineering (STITCH). By coupling STITCH with tetR linked to the KRAB domain to induce heterochromatin and disable the insulation, we developed a drug-...
ORGANISM(S): Homo sapiens 
4C-seq to show the effects of insertion of STITCH into NEUROG2-65kb positions on the chromatin conformation in neural progenitor cells differentiated from human iPSCs.
4C-seq to show the effects of insertion of STITCH into MYC+30kb and NEUROG2-65kb positions on the chromatin conformation in human iPSCs and differentiated neural progenitor cells
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