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ProteomicsQuantitative profiling of STOichiometry by Resolvable Mass tags
Post-translational modifications (PTMs) play important roles in modulating biological functions of proteins. Stoichiometry, which quantifies the modification percentage, is a critical factor for any given PTM. In this work, we developed a chemoproteomic strategy called “STO-MS” to systematically qua...
ORGANISM(S): Homo Sapiens 
PXD056632 |
ProteomicsAnalysis of acetylation stoichiometry suggests that SIRT3 repairs nonenzymatic acetylation lesions
Acetylation is frequently detected on mitochondrial enzymes and the sirtuin deacetylase SIRT3 is thought to regulate metabolism by deacetylating mitochondrial proteins. However, the stoichiometry of acetylation has not been studied and is important for understanding whether SIRT3 regulates or suppre...
ORGANISM(S): Mus musculus (Mouse) 
2015-09-15 | PXD000885 | Pride
Sirt3 Stoichiometry Acetylation Liver 
ProteomicsIntegrated lysine acetylation stoichiometry and proteomics analysis in human cells
Lysine acetylation is a widespread posttranslational modification that targets a large number of biological pathways. Recent studies reveal that lysine acetylation sites exhibit mainly low stoichiometry. Here we explored three sample preparation methods, the use of detergents for the chemical acetyl...
ORGANISM(S): Homo sapiens (Human) 
2017-09-12 | PXD005903 | Pride
Deacetylase inhibitor Sirt1 Cancer cells Lysine acetylation Stoichiometry Proteomics 
GenomicsAntarctica Stoichiometry
Antarctica Stoichiometry Targeted loci
PRJNA476992 | ENA
ProteomicsAnalysis of human acetylation stoichiometry defines mechanistic constraints on protein regulation
Lysine acetylation is a reversible posttranslational modification that occurs at thousands of sites on human proteins. However, the stoichiometry of acetylation remains poorly characterized, and is important for understanding acetylation-dependent mechanisms of protein regulation. Here we provide ac...
ORGANISM(S): Homo sapiens (Human) 
2019-03-12 | PXD009994 | Pride
Acetylation Acetylome Human Hela Stoichiometry 
ProteomicsDifferential stoichiometry among core ribosomal proteins
Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testi...
ORGANISM(S): Saccharomycetales (ncbitaxon:4892) Mus Musculus (ncbitaxon:10090) 
2015-08-28 | MSV000079280 | MassIVE
GenomicsAbsolute transcription-start nucleotide m6Am stoichiometry quantification by CROWN-Seq
Absolute transcription-start nucleotide m6Am stoichiometry quantification by CROWN-Seq
PRJNA977234 | ENA
GenomicsEvolutionary Convergence of Pathway-specific Enzyme Expression Stoichiometry
Evolutionary Convergence of Pathway-specific Enzyme Expression Stoichiometry
PRJNA376419 | ENA
ProteomicsAccurate quantification of site-specific acetylation stoichiometry reveals the impact of sirtuin deacetylase CobB on the E. coli acetylome
We applied SD-SILAC to determine absolute acetylation stoichiometry in exponentially-growing and stationary-phase wild type and sirtuin deacetylase CobB-deficient E. coli. We further assayed stoichiometry in phophotransacetylase (pta) and acetate kinase (ackA) mutant strains in the presence and abse...
ORGANISM(S): Escherichia coli 
2017-03-07 | PXD005757 | Pride
E. coli Sirtuin Acetylation Stoichiometry Cobb 
ProteomicsAccurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson’s disease
Pathogenic mutations in the Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson’s disease (PD). They increase its activity, resulting in augmented Rab10-Thr73 phosphorylation and conversely, LRRK2 inhibition decreases pRab10 levels. Currently, there is no assay to qua...
ORGANISM(S): Homo sapiens (Human) 
2020-07-03 | PXD019814 | Pride
Phosphorylation stoichiometry Targeted mass spectrometry Parkinson’s disease 
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