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scRNA-seq procedure and analysis. ScRNA-seq was performed using the Massively Parallel Single-Cell RNA-sequencing technology (MARS-seq) as described by Jaitin et al., 2014 (PMID: 24531970) EpiSCs were seeded at ~6 x 103 cells/cm2 in 6-well cell-culture plates pre-coated with 15 µg/ml human Fibronec...
ORGANISM(S): Mus musculus 
The experiment was designed to assess the DNA methylation differences between CLDN6Low vs CLDN6High EpiSCs and APSD, as well as WT vs Pbx1-KO EpiSCs. Based on analysis of RNAseq data and GO, we narrowed down our focus to DNA methylation effects influencing lineage biases. We performed WGBS analysi...
ORGANISM(S): Mus musculus 
RNAseq of CLDN6 sorted Epiblast Stem Cells (EpiSCs), WT vs Pbx1-KO lines in mouse embryonic stem cells (ES (2i/LIF and serum/LIF) and EpiSCs, as well as across differentiation (EpiSCs differentiated towards Posterior Primitive Streak (PPS) and Extraembryonic Mesoderm (ExM). Also EpiSCs treated with ...
ORGANISM(S): Mus musculus 
To explore the biological insights into squamous cell carcinoma (SCC) derived from different organs, we reported a deep proteomic analysis of 333 SCCs of 17 organs and 69 adenocarcinomas (ACs) of 7 organs. Proteomic comparison between pan-SCCs and pan-ACs identifies distinguishable pivotal pathways,...
ORGANISM(S): Homo Sapiens 
2022-05-19 | PXD033794 |
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