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UnknownAn improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells.
Not available
S-EPMC6033286 | biostudies-literature
ProteomicsRAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues, cancer cells, and macrophages - TMT MS RAPIDASH comparison with sucrose cushion of E14 mESCs
Ribosomes are emerging as direct regulators of gene expression, with ribosome-associated proteins (RAPs) allowing ribosomes to modulate translation. Nevertheless, a lack of technologies to enrich RAPs across sample types has prevented systematic analysis of RAP identities, dynamics, and functions. W...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-12 | PXD054298 | Pride
Acquity uplc m-class system Orbitrap elite Sucrose cushion Ribosome Tmt ms Ribosome-associated proteins Mouse 
UnknownSize-Exclusion Chromatography: A Path to Higher Yield and Reproducibility Compared to Sucrose Cushion Ultracentrifugation for Extracellular Vesicle Isolation in Multiple Myeloma.
Not available
S-EPMC11313515 | biostudies-literature
TranscriptomicsRibosome profiling with MN and RNaseI
The aim of this experiment is to compare nucleases used in ribosome profiling using Drosophila melanogaster Kc167 cells and human U2OS osteosarcoma cells. Ribosome profiling was performed from nuclease digested samples using sucrose cushion centrifugation separated ribosomes.
ORGANISM(S): Homo sapiens 
2015-01-08 | E-MTAB-2421 | biostudies-arrayexpress
TranscriptomicsAlzheimer's disease-related dysregulation of mRNA translation causes key pathological features with ageing
Primary rat cortical neurons and treated with AB for 24h. RNA-seq on total and ribosome-enriched sucrose cushion
ORGANISM(S): Rattus norvegicus 
2020-06-29 | GSE149540 | GEO
TranscriptomicsRiboSeq of BSR cells infected at MOI 3 with Theiler's murine encephalomyelitis virus, compared to mock-infected controls, harvested at 10 hpi. Fragments of lengths 35-65 nt purified.
BSR cells were infected with the Cardiovirus B archetype, Theiler’s murine encephalomyelitis virus (TMEV), and mutants thereof, and subjected to ribosome profiling to analyse ribosome collision events on the viral genome. Samples were harvested at 10 hpi by flash-freezing, without cycloheximide pre-...
ORGANISM(S): Mesocricetus auratus 
2021-11-10 | E-MTAB-9437 | biostudies-arrayexpress
TranscriptomicsRiboSeq of BSR cells infected at MOI 3 with Theiler's murine encephalomyelitis virus, compared to mock-infected controls, harvested at 10 hpi
BSR cells were infected with the Cardiovirus B archetype, Theiler’s murine encephalomyelitis virus (TMEV), and mutants thereof, and subjected to ribosome profiling to analyse the viral translatome, frameshifting, and ribosome pausing events. Samples were harvested at 10 hpi by flash-freezing, withou...
ORGANISM(S): Mesocricetus auratus 
2021-11-10 | E-MTAB-9438 | biostudies-arrayexpress
TranscriptomicsRiboSeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi, plus a control tunicamycin-treated sample.
Murine 17Cl-1 cells were infected with the model Betacoronavirus mouse hepatitis virus (MHV) strain A59 and subjected to ribosome profiling to observe changes in the host translatome in infected cells compared to mock-infected cells. Samples were harvested at 5 and 8 hpi by flash-freezing, without c...
ORGANISM(S): Mus musculus 
2021-05-18 | E-MTAB-8650 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV) strain, PE243
Vero cells were infected with the Asian/American Zika virus (ZIKV) strain, PE243, at MOI:3 to assess the viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was carried out, following which ribosome...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12971 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12969 | biostudies-arrayexpress
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