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Testing the effect of 80 small molecules on the interaction between 250 protein pairs
ORGANISM(S): Saccharomyces cerevisiae 
To understand the principles underlying protein-protein interaction (PPI) complex changes in response to external perturbations, we created a highly multiplexed version of the murine dihydrofolate reductase protein complementation assay (mDHFR PCA) in Saccharomyces cerevisiae, allowing quantitative ...
ORGANISM(S): Saccharomyces cerevisiae 
Background: The CRISPR/Cas9 toolbox has recently been expanded to include approaches for modulating gene expression. To successfully build on this work, and apply it for answering biological questions, it is important to establish it in a broad range of circumstances. Genome-scale CRISPR interferenc...
ORGANISM(S): Saccharomyces cerevisiae 
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