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The gene expression difference between CBA/J and DBA/2 mice after the injection of CAWS (Candida albicans water-soluble fraction) was compared using an oligo-DNA microarray. CAWS was injected into CBA/J and DBA/2 mice. 21 days after the injection, RNA was extracted from the splenocytes and measured ...
ORGANISM(S): Mus musculus 
BmMasc-GFP, BmMascNLS-GFP, or GFP was transiently expressed in BmN-4 cells seeded in 10 cm-diameter culture dishes. At three days post transfection, the cells were fixed with 0.1% formaldehyde, washed, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH (pH 7.5), 1 mM EGTA, 1 mM MgCl...
ORGANISM(S): Cellular Organisms 
Myelodysplastic syndromes (MDS) are clonal disorders of hematopoietic progenitors characterized by ineffective hematopoiesis and high propensity to leukemias. Although a number of gene targets have been identified, in many MDS cases, particular genetic targets are unknown. In this study, we performe...
ORGANISM(S): Homo sapiens 
OfMasc-GFP derivatives or GFP were transiently expressed in OfT1C/tet cells (3.2e6 cells) seeded in 10 cm-diameter culture dishes. At two days post transfection, the cells were fixed, collected, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 m...
ORGANISM(S): Cellular Organisms 
The accumulation of α-synuclein (ASyn) has been observed in several lysosomal storage diseases (LSD), but it remains unclear if the accumulation of ASyn contributes to the pathology. The mitochondria degeneration, reduced expression of manganese superoxide dismutase 2, and reactive oxygen species-me...
ORGANISM(S): Mus musculus 
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