RD3-KO Y-79 cells and RD3-SPOT-expressing cells were fixed by adding 27 µl of 37% formaldehyde (Wako) (final: 0.1%), incubated at RT for 10 min, quenched with 1 ml of 1 M Glycine-NaOH pH 7.5 (Nacalai) (final: 100 mM) at RT for 4 min, washed with 10 ml DPBS, and stocked at -80℃ as a cell pellet. For ...
ORGANISM(S): Homo Sapiens (human)
