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Proteomics‘One-pot’ sample processing method for proteome-wide analysis of microbial cells and spores
Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environme...
ORGANISM(S): Bacillus cereus (strain ATCC 14579 / DSM 31) Bacillus subtilis PY79 Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2018-07-05 | PXD008242 | Pride
Spores One-pot method Zic-hilic Proteomics Mass spectrometry 
ProteomicsIntegrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
Description Bacillus subtilis forms highly resistant, metabolically inactive spores upon nutrient limitation. These endospores pose challenges to the food and medical sectors. Spores reactivate their metabolism upon contact with germinants through germination and outgrowth, and then develop into ve...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-02 | MSV000085217 | MassIVE
ProteomicsInduced homogenous sporulation by kinA-overexpression leads to altered Bacillus subtilis spore proteomes and spore properties
Summary To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of sporulation synchronization on spore resistance, structures, the germination behavior at single-spore level and the proteome. ...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-28 | MSV000085346 | MassIVE
ProteomicsHigh resolution time-lapse proteomic analysis of sporulation
Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively inve...
ORGANISM(S): Bacillus Subtilis 
2021-04-03 | MSV000087154 | MassIVE
ProteomicsIdentification of Native Cross-links in Bacillus subtilis Spore Coat Proteins
The resistance properties of the bacterial spores are partially due to spore surface proteins, ~30% of which are said to form an insoluble protein fraction. Previous research has also identified a group of spore coat proteins affected by spore maturation, which exhibit an increased level of inter pr...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2021-01-09 | MSV000086681 | MassIVE
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