Syntrophus aciditrophicus is a model syntrophic bacterium that degrades fatty and aromatic acids into acetate, CO2, formate and H2 that are utilized by methanogens and other hydrogen-consuming microbes. The degradation of benzoate by S. aciditrophicus proceeds by a multi-step pathway that involves m...
We utilize a harsh mass-spectrometry compatible sample preparation method (eFASP), and label-free quantitative mass spectrometry, to collect a comprehensive inventory of proteins and the degree of differential regulation based on growth condition
Pure cultures of S. aciditrophicus grown in 250 ml of 20 mM crotonate medium in 500-ml Schott bottles were used for proteomic analysis after 2-dimensional (2-D) PAGE.
